Hinke Dekter

Tubes

Résumé

Experience

2018 - present

Account Manager

Boom BV, Meppel The Netherlands.

Function details:

  • Account manager
2010 - 2018

Laboratory Manager

Leiden Center for Bioscience, 
University of Applied Sciences Leiden

Function details:

  • Supervise research teams and interns
  • Organize daily operations of laboratory
  • Assist in all aspects of data collection (ISO-9001)
  • Quality officer, skilled with working with a LIMS system
  • Working according to ISO-17025 guidelines
  • Lead researcher in independent project
  • Teacher (University of Applied Sciences Leiden / Hogeschool Leiden)

Research projects:

Development of fast diagnostic tools in the field of (medical) Life Sciences, agro-culture and marinebiology

Top skills (in this function):

  • Laboratory management
  • Molecular Biology (PCR, RT-(q)PCT, Sequencing, DNA/RNA extraction etc)
  • Microbiology
  • Bio-physical methods (MALDI-TOF MS, Raman spectroscopy)
  • Immunology (ELISA,Western-blot, Immunoprecipitation)
2005 - 2010

Researcher

Department of Clinical Chemistry
Leiden University Medical Center (LUMC)

Research:

Development and validation of an ELISA-BASED assay to measure the mTOR activity in renal allograft patients.

Techniques:

  • Development and optimalization of ELISA based assay’s
  • SDS-PAGE
  • Western-Blot
  • Cell culture (human cells)
  • Isolation of cells from full blood
  • FACS analysis
  • Confocal laser scanning microscopy and conventional fluorescence microscopy
  • Immunochemical localization
2004 - 2005

Researcher (internship)

Department of Orthopaedics
University Mecical Center Utrecht (UMCU)

Research

siRNA-mediated inhibition of matrix degrading enzymes in osteoarthritic chondrocytes.

Techniques

  • SDS-PAGE
  • Western-Blot
  • Cell culture (human cells, animal cells, transfection etc)
  • Isolation of chondrocytes from human cartilage samples
  • Confocal laser scanning microscopy and conventional fluorescence microscopy
  • siRNA synthese
  • siRNA transfection
  • Immunochemical localization
2003 - 2004

Researcher (internship)

Department Otolaryngology/Head and neck surgery, section Tumorbiology
VU University Medical Center Amsterdam

Research:

Head and neck carcinogenesis of two special patient populations; Fanconi anemia patients and young adults.

Techniques:

  • Radio-isotopic techniques (p53 sequencer)
  • DNA extraction and purification from histological coupes
  • Micro-dissection
  • Electrophoresis
  • Labelling of DNA (radioactive en fluorescent)
  • PCR
  • Microsattelite analysis (ABIPRISM sequencer)
  • P53 mutation-analysis

Researcher (internship)

2002 – 2003
Department applied tumor virology (ATV)
Krebsforschungszentrum (Heidelberg, Germany)

Research:

Characterization of CML66 an interaction partner of the HPV11-L2 protein.

Techniques:

  • SDS-PAGE
  • recombinant DNA techniques
  • Western-Blot
  • Cell culture (human cells, animal cells, transfection etc)
  • RNA- en DNA isolation
  • Electrophoresis
  • Cloning
  • Confocal laser scanning microscopy
1999-2000

Researcher (internship)

Department of pathology
St. Anthonius Hospital (Nieuwegein, NL)

Research:

Finding the best colouring techniques for cytological coupes. Furthermore a traineeship in histological techniques.

Techniques:

  • Preparing histological coupes (paraffine, freeze and plastic)
  • Preparing cytological coupes
  • Colouring techniques histology and cytology

Education

2003 - 2005

Biomedical Sciences, Vrije Universiteit Amsterdam

2000 - 2003

Molecular Biology, University of Applied Sciences Leiden

1997 - 2000

Pathology, ROC de Amerlanden

Extra curricular classes

Radio isotopic techniques (VU University Medical Center Amsterdam)
Veilige Microbiologische Technieken (VMT; University of Applied Sciences Leiden
Biostatistics (Leiden University Medical Center)
Bionumerics (Applied Math)

Computer skills

Microsoft Office Suite (Access, Excel, Outlook, PowerPoint, Word, Graphpad)
SPSS
ABI Prism software / GeneScan Analysis
Bionumerics
LIMS (Scienz)

Languages

Dutch: native
English: fluent
German: moderate